Redistribution of DNA loop domains in human lymphocytes under blast transformation with interleukin 2 [Text] = Перерозподіл петельних доменів ДНК у лімфоцитах людини у разі бласттрансформації за впливу інтерлейкіну 2 / K. S. Afanasieva [и др.] // Український біохімічний журнал. - 2016. - Т. 88, № 6. - P45-51. - Bibliogr. at the end of the art.
MeSH-главная:
ТРАНСКРИПЦИЮ АКТИВИРУЮЩИЕ ФАКТОРЫ -- ACTIVATING TRANSCRIPTION FACTORS (анализ)
ЛИМФОЦИТЫ -- LYMPHOCYTES (действие лекарственных препаратов, ультраструктура)
ИНТЕРЛЕЙКИН-2 -- INTERLEUKIN-2 (анализ, физиология)
ДНК-КОМЕТ МЕТОД -- COMET ASSAY (методы, статистика)
КЛЕТКИ ЯДРО -- CELL NUCLEUS (ультраструктура, физиология)
Аннотация: At higher order levels chromatin fibers in interphase nuclei are organized into loop domains. Gene regulatory elements (promoters and enhancers) are often located near the sites of loop attachments. Therefore, loop domains play a key role in regulation of cell transcriptional activity. We investigated the kinetics of DNA loop exit during single cell gel electrophoresis (the comet assay) of nucleoids obtained from two cell types that differ in their synthetic activity – human lymphocytes and lymphoblasts. Lymphocyte activation and transformation into lymphoblasts (blast transformation) was performed with interleukin 2. The results obtained suggest that a rearrangement of the loops occurs after lymphocyte activation. After blast transformation we observed an increase of the amount of loop domains on the surface of nucleoids against a decrease of the inner loop fraction. Therefore, the comet assay can be used for detection of large-scale changes in the cell nucleus that follow changes in cell functional state
Доп.точки доступа:
Afanasieva, K. S.; Chopei, M. I.; Lozovik, A. V.; Rushkovsky, S. R.; Sivolob, A. V.
Экз-ры:
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