Volkova, N. O.     Potentiation of cryopreserved rat adipose-derived multipotent mesenchymal stromal cells by BMP-12 in vitro for the treatment of tendinopathy [] / N. O. Volkova, M. S. Yukhta, A. M. Goltsev // Клітинна та органна трансплантологія. - 2018. - Том 6, N 2. - С. 131-136. - Bibliogr. at the end of the art. MeSH-главная: ТЕНДИНОПАТИЯ -- TENDINOPATHY (патофизиология, этиология) ТРАНСФОРМИРУЮЩИЙ ФАКТОР РОСТА БЕТА -- TRANSFORMING GROWTH FACTOR BETA (анализ) МОДЕЛИ НА ЖИВОТНЫХ -- MODELS, ANIMAL КРИОПРЕЗЕРВАЦИЯ -- CRYOPRESERVATION (использование, методы) СТРОМАЛЬНЫЕ КЛЕТКИ МЕЗЕНХИМНЫЕ -- MESENCHYMAL STROMAL CELLS КЛЕТОЧНАЯ И ТКАНЕВАЯ ТЕРАПИЯ -- CELL- AND TISSUE-BASED THERAPY (использование, методы, тенденции) ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS Аннотация: Bone morphogenetic proteins (BMP), which are part of TGF-b superfamily, have a stimulating effect on bone formation, as well as the proliferation oftenocytes and fibroblasts, which form the tendon tissue The PURPOSE of the study was to determine the effect of BMP-12 on the morphofunctional characteristics of cryopreserved rat adipose tissue- derived multipotent mesenchymal stromal cells (AT-MMSCs) and to investigate the effects of the use ofAT-MMSCs pre-cultured with BMP-12 on the tendinopathy model MATERIALS AND METHODS. MMSCs were obtained from the adipose tissue of rats. Cryopreservation was carried out under the protection of 10 % DMSO with the addition of 20 % fetal bovine serum at a cooling rate of 1 °C/min to -80 °С and subsequent transfer to liquid nitrogen. When culturing AT-MMSCs, the BMP-12 was added at the concentration of 50 ng/mL. The ability of cells to proliferation (by MTT-test) and the synthesis of collagen I and III types were evaluated. Rats with modeled degenerative-dystrophic injury to the Achilles tendons were locally administered with 0.25-1 № AT-MMSCs orAT-MMSCs+BMP-12 in the defect zone. The animals injected with saline provided control group. On the 21st day after the treatment, a histological, immunofluorescence and biomechanical studies were performed RESULTS. The application of BMP-12 results in the decrease in proliferation ofAT-MMSCs along with an increase in the relative number of cells that synthesize collagen I and III types relative to AT-MMSCs cultivated under standard conditions. The injection ofAT-MMSCs promotes the activation of regenerative processes in damaged tendons. The use of AT-MMSCs+BMP-12 accelerates the histological structure, strength, and the content of collagen I and III types in the Achilles tendons of animals with degenerative-dystrophic damage compared to the cells without BMP-12 CONCLUSIONS BMP-12 can be used as a stimulating agent for the tenogenic differentiation ofAT-MMSCs before transplantation into the damaged tendon tissue Доп.точки доступа: Yukhta, M.S.; Goltsev, A. M. Экз-ры: