Вид документа : Статья из журнала Шифр издания : Автор(ы) : Kaplia A. A. Заглавие : The influence of heavy metal ions, spermine and sodium nitroprusside on ATP-hydrolases of cell membranes of rat colon smooth muscle Параллельн. заглавия :Вплив іонів важких металів, сперміну та нітропрусиду натрію на АТР-гідролази клітинних мембран гладеньких м’язів ободової кишки щура Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 4. - С. 20-28 (Шифр УУ1/2016/88/4) Примечания : Bibliogr. at the end of the art. MeSH-главная: МОДЕЛИ НА ЖИВОТНЫХ -- MODELS, ANIMAL МЫШЦА ГЛАДКАЯ -- MUSCLE, SMOOTH МЕТАЛЛЫ ТЯЖЕЛЫЕ -- METALS, HEAVY ОБОДОЧНАЯ КИШКА -- COLON ГИДРОЛАЗЫ -- HYDROLASES КЛЕТОЧНАЯ МЕМБРАНА -- CELL MEMBRANE Аннотация: The specific features of functional lability of the rat colon smooth muscle (CSM) АТР-hydrolases were studied. Na[[p]]+[[/p]],K[[p]]+[[/p]]-ATPase activity is effectively inhibited by divalent ions of both transition ( 0,1 ??) and nontransition ( 1 ??) heavy metals in succession by efficiency: Cu[[p]]2+[[/p]] Fe[[p]]2+[[/p]] Cd[[p]]2+[[/p]] (10 ??). Polyamine spermine (0,5-1,0 mM) is a weak Na[[p]]+[[/p]],K[[p]]+[[/p]]-ATPase inhibitor at saturation concentrations of ions and substrate. Sodium nitroprusside (1 mM) as nitric oxide-generating compound exhibits weak Na[[p]]+[[/p]],K[[p]]+[[/p]]-ATPase inhibition only after prolonged preincubation with membranes. Mg[[p]]2+[[/p]]-ATP-hydrolase activity in all cases is much more resistant to studied agents. Considering the example of the CSM Na[[p]]+[[/p]],K[[p]]+[[/p]]-ATPase it is assumed that enzyme has specific biochemical features that contribute to its role as a potential target and redox-sensor, mediating the pathological mechanisms of heavy metal intoxication and cell oxidative damage